working of hplc system Fundamentals Explained
To circumvent the lack of stationary phase, which shortens the column’s lifetime, it can be certain covalently for the silica particles. Bonded stationary phasesIf we switch from using acetonitrile to tetrahydrofuran, one example is, we learn that benzoic acid elutes more swiftly Which p-hydroxybenzoic acid elutes much more slowly and gradually.